MP-SPR for biosensor development
MP-SPR is an invaluable tool for assay development in fields such as food and feed safety, environmental safety, clinical diagnostics, border control and process control. While part of the research worldwide is dedicated to high-throughput instruments, more and more attention is gained by portable or point-of-care instruments. MP-SPR is an excellent tool for development of portable biosensors. Point-of-care biosensors include typically electrochemical sensors, surface enhance Raman sensors (SERS), ELISA, fluorescence or newly also printed diagnostics.
- Easy ex-situ modification of SPR surfaces
- Any assay type
- Any sample
- Easy validation
- Microfluidic material testing
For information on electrochemical biosensor development click here.
See five key questions in assay development that MP-SPR can answer:
How fast do the molecules X bind with surface A as compared to surface B?
What is the optimum surface for my biosensor applications?
How does my assay work with crude sample such as serum, saliva, sea water or urine?
Is the biosensor that I have designed working better than the previous one?
Which coating prevents sample adsorption onto my microfluidic channels?
For electrochemical sensor development using MP-SPR, click here.
Printed biosensor development using MP-SPR:
MEMS sensor development using MP-SPR:
Novel coatings to avoid non-specific binding using MP-SPR:
- Surface Plasmon Resonance on Nanoscale Organic Films, Albers MW and Vikholm-Lundin I, Nano-Bio-Sensing, 1st ed, Chapter 4, Springer 2010
- Grafting hyaluronic acid onto gold surface to achieve low protein fouling in surface plasmon resonance biosensors, Liu X et al, ACS Appl Mater Interfaces. 2014 Aug 13;6(15):13034-42
- Effects of Composition of Oligo(ethylene glycol)-Based Mixed Monolayers on Peptide Grafting and Human Immunoglobulin Detection, Islam N et al., Journal of Physical Chemistry, 2014, 118 (10), pp 5361–5373
Assay development using MP-SPR:
- Dynamic and equilibrium performance of sensors based on short peptide ligands for affinity adsorption of human IgG using surface plasmon resonance, Islam N et al., Biosensors and Bioelectronics, 58, 2014, p. 380-387
- Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor, Zeng C et al., Anal. Biochem. 440 (2013) 18–22
- Development of diagnostic SPR based biosensor for the detection of pharmaceutical compounds in saliva, Sonny S et al., Proc. SPIE 7376, Laser Applications in Life Sciences, 737605
- Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the anti-tumor immunity and efficacy against melanoma, Capasso et al., Taylor & Francis Online, 2015
Improving performance of optical biosensors: