MP-SPR用于活细胞

基于细胞的研究背后有许多驱动因素，包括组织工程、体外毒理学、药物递送以及抗菌涂层。
表面等离子共振分析仪最初用于蛋白质相互作用的测量。直到最近，研究人员才开始开发SPR用于测量活体细胞。我们很自豪成为第一家能够测量跨细胞和细胞旁摄取的SPR制造商。
BioNavis和赫尔辛基大学一起开创了药物和纳米粒子-细胞相互作用的测量。我们的许多用户使用MP-SPR测量细胞在不同生物材料和涂层上的扩散和附着。MP-SPR还可用作检测细胞特定代谢产物的生物传感器，以确认细胞分化和/或细胞功能。同样，一些小组正在研究生物膜的形成或抗菌涂料。

选择MP-SPR用于活细胞的五个原因：

1、能够实时测量活细胞和细菌附着

2、能够无标记区分渗透和内化
3、表面自由度
4、温度和剪应力控制
5、MP-SPR与电化学的结合

MP-SPR可以回答的关于活细胞的五个关键问题：

1、这种药物的药物吸收途径是什么？
2、哪种纳米粒子最适合药物输送？
3、纳米粒子或病毒是如何进入细胞的？
4、细胞附着在表面的动力学是多少？
5、哪种表面对细菌生长最具抵抗力？

BioNavis_AN#137_使用MP-SPR测量药物与细胞单层的相互作用

BioNavis_AN#145_使用MP-SPR研究病毒之间的相互作用

BioNavis_AN#154_使用MP-SPR测量植入材料表面癌细胞的实时检测与粘附

BioNavis_AN#156_用MP-SPR测量细胞对纳米颗粒的摄入

BioNavis_AN#160_生物传感器用于检测奶粉中的细菌

Selected publications:

• Endothelial cells' biophysical, biochemical, and chromosomal aberrancies in high ‐ glucose condition within the diabetic range, Cell Biochemistry & Function, 2017, p. 1-15
• Feasibility Study of the Permeability and Uptake of Mesoporous Silica Nanoparticles across the Blood-Brain Barrier, PLoS ONE 11(8): e0160705, 2016
• Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake, Small, 2016
• Real-Time Protein and Cell Binding Measurements on Hydroxyapatite Coatings, J. of Functional Biomaterials, 2016, Vol. 7(3), p. 23
• Elucidating the Signal Responses of Multi-Parametric Surface Plasmon Resonance Living Cell Sensing: A Comparison between Optical Modeling and Drug–MDCKII Cell Interaction Measurements, PLoS ONE 8(8), 2013, e72192
• Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells, Biomaterials, Volume 1, May 2015, Pages 257-269
• Structural and Viscoelastic Properties of Layer-by-Layer Extracellular Matrix (ECM) Nanofilms and Their Interactions with Living Cell, ACS Biomater. Sci. Engineering, 2015, 1[9]: p. 816–824
• Microbial attachment and adsorption–desorption kinetic of tightly bound extracellular polymeric substances on model organic surfaces, Chemical Engineering Journal, 2015, 279: p. 516–521
• Investigation of cell behaviors on thermo-responsive PNIPAM microgel films, Colloids and Surfaces B: Biointerfaces, 132: p. 202–207
• Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility,  J. of Biomed. Mat. Research Part A, 2013, 101A, 413–420
• Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the anti-tumor immunity and efficacy against melanoma, Capasso et al., Taylor & Francis Online, 2015